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KMID : 0380219940270040313
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 4 p.313 ~ p.315
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In Vitro Protein Translocation into Inverted Membrane Vesicles Prepared from Vibrio Alginolyticus and the Role of a Na+ Electrochemical Potential
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Abstract
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Abstract:
@EN The role of a Na+ electrochemical potential on protein translocation was examined. The translocation of E. coli uncleavable OmpF-Lpp into inverted membrane vesicles prepared from Na+ pump-containing V. alginolyticus was little inhibited in
the
presence of the proton conductor, carbonyl cyanide m-chlorophenylhydrazone(CCCP). In contrast, monensin, which dissipates a Na+ electrochemical potential, significantly inhibited the translocation of uncleavable OmpF-Lpp to the level of
translocation
activity observed in the absence of NADH. These results suggest that the electrochemical potential of Na+, but not that of H+, plays an important role in protein translocation in V. alginolyticus.
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KEYWORD
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